ASD-GFP vectors for in vivo expression technology in Pseudomonas aeruginosa and other gram-negative bacteria

M. Handfield; H. P. Schweizer; M. J. Mahan; F. Sanschagrin; T. Hoang; R. C. Levesque

doi:10.2144/98242st02

ASD-GFP vectors for in vivo expression technology in Pseudomonas aeruginosa and other gram-negative bacteria

M. Handfield, H. P. Schweizer, M. J. Mahan, F. Sanschagrin, T. Hoang, R. C. Levesque

Research output: Contribution to journal › Article › peer-review

18 Scopus citations

Abstract

We describe the construction of promoter probe vectors designed for identification of bacterial genes induced in vitro and/or in vivo and for measurement of gene expression levels for in vivo expression technology. These plasmids use the Pseudomonas aeruginosa aspartate β-semialdehyde dehydrogenase (asd) gene as a selectable marker and β-galactosidase (pIVPRO, 10.88 kb) or mutant green fluorescent protein (mut3GFP, pIVET-GFP, 5.48 kb) as reporter gene systems. The proposed strategies can be adapted for use in most Gram-negative bacteria.

Original language	English (US)
Pages (from-to)	261-264
Number of pages	4
Journal	BioTechniques
Volume	24
Issue number	2
DOIs	https://doi.org/10.2144/98242st02
State	Published - 1998
Externally published	Yes

ASJC Scopus subject areas

Biotechnology
General Biochemistry, Genetics and Molecular Biology

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abstract = "We describe the construction of promoter probe vectors designed for identification of bacterial genes induced in vitro and/or in vivo and for measurement of gene expression levels for in vivo expression technology. These plasmids use the Pseudomonas aeruginosa aspartate β-semialdehyde dehydrogenase (asd) gene as a selectable marker and β-galactosidase (pIVPRO, 10.88 kb) or mutant green fluorescent protein (mut3GFP, pIVET-GFP, 5.48 kb) as reporter gene systems. The proposed strategies can be adapted for use in most Gram-negative bacteria.",

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AU - Handfield, M.

AU - Schweizer, H. P.

AU - Mahan, M. J.

AU - Sanschagrin, F.

AU - Hoang, T.

AU - Levesque, R. C.

PY - 1998

Y1 - 1998

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AB - We describe the construction of promoter probe vectors designed for identification of bacterial genes induced in vitro and/or in vivo and for measurement of gene expression levels for in vivo expression technology. These plasmids use the Pseudomonas aeruginosa aspartate β-semialdehyde dehydrogenase (asd) gene as a selectable marker and β-galactosidase (pIVPRO, 10.88 kb) or mutant green fluorescent protein (mut3GFP, pIVET-GFP, 5.48 kb) as reporter gene systems. The proposed strategies can be adapted for use in most Gram-negative bacteria.

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ASD-GFP vectors for in vivo expression technology in Pseudomonas aeruginosa and other gram-negative bacteria

Abstract

ASJC Scopus subject areas

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