An improved model for the hTERT promoter quadruplex

Jonathan B. Chaires; John O. Trent; Robert D. Gray; William L. Dean; Robert Buscaglia; Shelia D. Thomas; Donald M. Miller

doi:10.1371/journal.pone.0115580

An improved model for the hTERT promoter quadruplex

Jonathan B. Chaires, John O. Trent, Robert D. Gray, William L. Dean, Robert Buscaglia, Shelia D. Thomas, Donald M. Miller

Research output: Contribution to journal › Article › peer-review

52 Scopus citations

Abstract

Mutations occur at four specific sites in the hTERT promoter in >75% of glioblastomas and melanomas, but the mechanism by which the mutations affect gene expression remains unexplained. We report biophysical computational studies that show that the hTERT promoter sequence forms a novel G-quadruplex structure consisting of three contiguous, stacked parallel quadruplexes. The reported hTERT mutations map to the central quadruplex within this structure, and lead to an alteration of its hydrodynamic properties and stability.

Original language	English (US)
Article number	e115580
Journal	PLoS ONE
Volume	9
Issue number	12
DOIs	https://doi.org/10.1371/journal.pone.0115580
State	Published - Dec 19 2014
Externally published	Yes

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abstract = "Mutations occur at four specific sites in the hTERT promoter in >75% of glioblastomas and melanomas, but the mechanism by which the mutations affect gene expression remains unexplained. We report biophysical computational studies that show that the hTERT promoter sequence forms a novel G-quadruplex structure consisting of three contiguous, stacked parallel quadruplexes. The reported hTERT mutations map to the central quadruplex within this structure, and lead to an alteration of its hydrodynamic properties and stability.",

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AU - Chaires, Jonathan B.

AU - Trent, John O.

AU - Gray, Robert D.

AU - Dean, William L.

AU - Buscaglia, Robert

AU - Thomas, Shelia D.

AU - Miller, Donald M.

PY - 2014/12/19

Y1 - 2014/12/19

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AB - Mutations occur at four specific sites in the hTERT promoter in >75% of glioblastomas and melanomas, but the mechanism by which the mutations affect gene expression remains unexplained. We report biophysical computational studies that show that the hTERT promoter sequence forms a novel G-quadruplex structure consisting of three contiguous, stacked parallel quadruplexes. The reported hTERT mutations map to the central quadruplex within this structure, and lead to an alteration of its hydrodynamic properties and stability.

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An improved model for the hTERT promoter quadruplex

Abstract

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