An improved model for the hTERT promoter quadruplex

Jonathan B. Chaires, John O. Trent, Robert D. Gray, William L. Dean, Robert Buscaglia, Shelia D. Thomas, Donald M. Miller

Research output: Contribution to journalArticlepeer-review

39 Scopus citations

Abstract

Mutations occur at four specific sites in the hTERT promoter in >75% of glioblastomas and melanomas, but the mechanism by which the mutations affect gene expression remains unexplained. We report biophysical computational studies that show that the hTERT promoter sequence forms a novel G-quadruplex structure consisting of three contiguous, stacked parallel quadruplexes. The reported hTERT mutations map to the central quadruplex within this structure, and lead to an alteration of its hydrodynamic properties and stability.

Original languageEnglish (US)
Article numbere115580
JournalPLoS ONE
Volume9
Issue number12
DOIs
StatePublished - Dec 19 2014
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)
  • General

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