Activity-dependent fluorescent labeling of bacterial cells expressing the TOL pathway

Scott R. Clingenpeel; William K. Keener; Caron R. Keller; Karl De Jesus; M. Hope Howard; Mary E. Watwood

doi:10.1016/j.mimet.2004.08.018

Activity-dependent fluorescent labeling of bacterial cells expressing the TOL pathway

Scott R. Clingenpeel
, William K. Keener
, Caron R. Keller
, Karl De Jesus
, M. Hope Howard
, Mary E. Watwood

Biological Sciences

Research output: Contribution to journal › Article › peer-review

8 Scopus citations

Abstract

3-Ethynylbenzoate (3EB) functions as a novel, activity-dependent, fluorogenic, and chromogenic probe for bacterial strains expressing the TOL pathway, which degrade toluene via conversion to benzoate, followed by meta ring fission of the intermediate catechol. This direct physiological analysis allows the fluorescent labeling of cells whose toluene-degrading enzymes have been induced by an aromatic substrate.

Original language	English (US)
Pages (from-to)	41-46
Number of pages	6
Journal	Journal of Microbiological Methods
Volume	60
Issue number	1
DOIs	https://doi.org/10.1016/j.mimet.2004.08.018
State	Published - Jan 2005

Keywords

3-Ethynylbenzoate
Bioremediation
Chromogenic
Fluorogenic
Toluene

ASJC Scopus subject areas

Microbiology
Molecular Biology
Microbiology (medical)

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10.1016/j.mimet.2004.08.018

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title = "Activity-dependent fluorescent labeling of bacterial cells expressing the TOL pathway",

abstract = "3-Ethynylbenzoate (3EB) functions as a novel, activity-dependent, fluorogenic, and chromogenic probe for bacterial strains expressing the TOL pathway, which degrade toluene via conversion to benzoate, followed by meta ring fission of the intermediate catechol. This direct physiological analysis allows the fluorescent labeling of cells whose toluene-degrading enzymes have been induced by an aromatic substrate.",

keywords = "3-Ethynylbenzoate, Bioremediation, Chromogenic, Fluorogenic, Toluene",

author = "Clingenpeel, \{Scott R.\} and Keener, \{William K.\} and Keller, \{Caron R.\} and \{De Jesus\}, Karl and Howard, \{M. Hope\} and Watwood, \{Mary E.\}",

note = "Funding Information: This work was supported by the Inland Northwest Research Alliance through a grant to M. Watwood and a graduate fellowship to S. Clingenpeel. Support was also provided from the INEEL and the DOE EPSCoR program.",

year = "2005",

month = jan,

doi = "10.1016/j.mimet.2004.08.018",

language = "English (US)",

volume = "60",

pages = "41--46",

journal = "Journal of Microbiological Methods",

issn = "0167-7012",

publisher = "Elsevier B.V.",

number = "1",

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TY - JOUR

T1 - Activity-dependent fluorescent labeling of bacterial cells expressing the TOL pathway

AU - Clingenpeel, Scott R.

AU - Keener, William K.

AU - Keller, Caron R.

AU - De Jesus, Karl

AU - Howard, M. Hope

AU - Watwood, Mary E.

N1 - Funding Information: This work was supported by the Inland Northwest Research Alliance through a grant to M. Watwood and a graduate fellowship to S. Clingenpeel. Support was also provided from the INEEL and the DOE EPSCoR program.

PY - 2005/1

Y1 - 2005/1

N2 - 3-Ethynylbenzoate (3EB) functions as a novel, activity-dependent, fluorogenic, and chromogenic probe for bacterial strains expressing the TOL pathway, which degrade toluene via conversion to benzoate, followed by meta ring fission of the intermediate catechol. This direct physiological analysis allows the fluorescent labeling of cells whose toluene-degrading enzymes have been induced by an aromatic substrate.

AB - 3-Ethynylbenzoate (3EB) functions as a novel, activity-dependent, fluorogenic, and chromogenic probe for bacterial strains expressing the TOL pathway, which degrade toluene via conversion to benzoate, followed by meta ring fission of the intermediate catechol. This direct physiological analysis allows the fluorescent labeling of cells whose toluene-degrading enzymes have been induced by an aromatic substrate.

KW - 3-Ethynylbenzoate

KW - Bioremediation

KW - Chromogenic

KW - Fluorogenic

KW - Toluene

UR - https://www.scopus.com/pages/publications/9644257120

UR - https://www.scopus.com/pages/publications/9644257120#tab=citedBy

U2 - 10.1016/j.mimet.2004.08.018

DO - 10.1016/j.mimet.2004.08.018

M3 - Article

C2 - 15567223

AN - SCOPUS:9644257120

SN - 0167-7012

VL - 60

SP - 41

EP - 46

JO - Journal of Microbiological Methods

JF - Journal of Microbiological Methods

IS - 1

ER -

Activity-dependent fluorescent labeling of bacterial cells expressing the TOL pathway

Abstract

Keywords

ASJC Scopus subject areas

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