Activity-dependent fluorescent labeling of bacterial cells expressing the TOL pathway

Scott R. Clingenpeel, William K. Keener, Caron R. Keller, Karl De Jesus, M. Hope Howard, Mary E. Watwood

Research output: Contribution to journalArticlepeer-review

8 Scopus citations


3-Ethynylbenzoate (3EB) functions as a novel, activity-dependent, fluorogenic, and chromogenic probe for bacterial strains expressing the TOL pathway, which degrade toluene via conversion to benzoate, followed by meta ring fission of the intermediate catechol. This direct physiological analysis allows the fluorescent labeling of cells whose toluene-degrading enzymes have been induced by an aromatic substrate.

Original languageEnglish (US)
Pages (from-to)41-46
Number of pages6
JournalJournal of Microbiological Methods
Issue number1
StatePublished - Jan 2005


  • 3-Ethynylbenzoate
  • Bioremediation
  • Chromogenic
  • Fluorogenic
  • Toluene

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology
  • Microbiology (medical)


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