A rapid DNA extraction method for sugarcane and its relatives

Rhonda J. Honeycutt; Bruno W.S. Sobral; Paul Keim; James E. Irvine

doi:10.1007/BF02669266

A rapid DNA extraction method for sugarcane and its relatives

Rhonda J. Honeycutt, Bruno W.S. Sobral, Paul Keim, James E. Irvine

Biological Sciences

Research output: Contribution to journal › Article › peer-review

32 Scopus citations

Abstract

A simple DNA extraction method based on CTAB precipitation was used to obtain DNA from members of the genus Saccharum and related species. DNA yields and purities were similar for all Saccharum species sampled. The method described here resulted in high quality total DNA suitable for polymerase chain reaction (PCR)-based techniques as well as restriction endonuclease digestion, Southern hybridization, and DNA cycle-sequencing.

Original language	English (US)
Pages (from-to)	66-72
Number of pages	7
Journal	Plant Molecular Biology Reporter
Volume	10
Issue number	1
DOIs	https://doi.org/10.1007/BF02669266
State	Published - Feb 1992

Keywords

Andropogoneae
DNA extraction
Saccharum complex
grasses
sugarcane

ASJC Scopus subject areas

Molecular Biology
Plant Science

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10.1007/BF02669266

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title = "A rapid DNA extraction method for sugarcane and its relatives",

abstract = "A simple DNA extraction method based on CTAB precipitation was used to obtain DNA from members of the genus Saccharum and related species. DNA yields and purities were similar for all Saccharum species sampled. The method described here resulted in high quality total DNA suitable for polymerase chain reaction (PCR)-based techniques as well as restriction endonuclease digestion, Southern hybridization, and DNA cycle-sequencing.",

keywords = "Andropogoneae, DNA extraction, Saccharum complex, grasses, sugarcane",

author = "Honeycutt, {Rhonda J.} and Sobral, {Bruno W.S.} and Paul Keim and Irvine, {James E.}",

year = "1992",

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AU - Honeycutt, Rhonda J.

AU - Sobral, Bruno W.S.

AU - Keim, Paul

AU - Irvine, James E.

PY - 1992/2

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AB - A simple DNA extraction method based on CTAB precipitation was used to obtain DNA from members of the genus Saccharum and related species. DNA yields and purities were similar for all Saccharum species sampled. The method described here resulted in high quality total DNA suitable for polymerase chain reaction (PCR)-based techniques as well as restriction endonuclease digestion, Southern hybridization, and DNA cycle-sequencing.

KW - Andropogoneae

KW - DNA extraction

KW - Saccharum complex

KW - grasses

KW - sugarcane

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A rapid DNA extraction method for sugarcane and its relatives

Abstract

Keywords

ASJC Scopus subject areas

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