A rapid DNA extraction method for sugarcane and its relatives

Rhonda J. Honeycutt, Bruno W.S. Sobral, Paul Keim, James E. Irvine

Research output: Contribution to journalArticlepeer-review

31 Scopus citations


A simple DNA extraction method based on CTAB precipitation was used to obtain DNA from members of the genus Saccharum and related species. DNA yields and purities were similar for all Saccharum species sampled. The method described here resulted in high quality total DNA suitable for polymerase chain reaction (PCR)-based techniques as well as restriction endonuclease digestion, Southern hybridization, and DNA cycle-sequencing.

Original languageEnglish (US)
Pages (from-to)66-72
Number of pages7
JournalPlant Molecular Biology Reporter
Issue number1
StatePublished - Feb 1992


  • Andropogoneae
  • DNA extraction
  • Saccharum complex
  • grasses
  • sugarcane

ASJC Scopus subject areas

  • Molecular Biology
  • Plant Science


Dive into the research topics of 'A rapid DNA extraction method for sugarcane and its relatives'. Together they form a unique fingerprint.

Cite this