A high resolution four-locus multiplex single nucleotide repeat (SNR) genotyping system in Bacillus anthracis

L. J. Kenefic; J. Beaudry; C. Trim; L. Huynh; S. Zanecki; M. Matthews; J. Schupp; M. Van Ert; P. Keim

doi:10.1016/j.mimet.2007.11.014

A high resolution four-locus multiplex single nucleotide repeat (SNR) genotyping system in Bacillus anthracis

L. J. Kenefic
, J. Beaudry
, C. Trim
, L. Huynh
, S. Zanecki
, M. Matthews
, J. Schupp
, M. Van Ert
, P. Keim

Biological Sciences

Research output: Contribution to journal › Article › peer-review

35 Scopus citations

Abstract

The allelic identities of Single Nucleotide Repeat (SNR) markers in Bacillus anthracis are typically ascertained by DNA sequencing through the direct repeat. Here we describe a reproducible method for genotyping closely related isolates by using four SNR loci in a multiplex-PCR capillary electrophoresis system amenable to high-throughput analysis.

Original language	English (US)
Pages (from-to)	269-272
Number of pages	4
Journal	Journal of Microbiological Methods
Volume	73
Issue number	3
DOIs	https://doi.org/10.1016/j.mimet.2007.11.014
State	Published - Jun 2008

Keywords

Bacillus anthracis
Capillary electrophoresis
MLVA
SNR

ASJC Scopus subject areas

Microbiology
Molecular Biology
Microbiology (medical)

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10.1016/j.mimet.2007.11.014

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title = "A high resolution four-locus multiplex single nucleotide repeat (SNR) genotyping system in Bacillus anthracis",

abstract = "The allelic identities of Single Nucleotide Repeat (SNR) markers in Bacillus anthracis are typically ascertained by DNA sequencing through the direct repeat. Here we describe a reproducible method for genotyping closely related isolates by using four SNR loci in a multiplex-PCR capillary electrophoresis system amenable to high-throughput analysis.",

keywords = "Bacillus anthracis, Capillary electrophoresis, MLVA, SNR",

author = "Kenefic, \{L. J.\} and J. Beaudry and C. Trim and L. Huynh and S. Zanecki and M. Matthews and J. Schupp and \{Van Ert\}, M. and P. Keim",

note = "Funding Information: We would like to thank the Northern Arizona University Cowden Endowment for Microbiology for support of this research.",

year = "2008",

month = jun,

doi = "10.1016/j.mimet.2007.11.014",

language = "English (US)",

volume = "73",

pages = "269--272",

journal = "Journal of Microbiological Methods",

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publisher = "Elsevier B.V.",

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TY - JOUR

T1 - A high resolution four-locus multiplex single nucleotide repeat (SNR) genotyping system in Bacillus anthracis

AU - Kenefic, L. J.

AU - Beaudry, J.

AU - Trim, C.

AU - Huynh, L.

AU - Zanecki, S.

AU - Matthews, M.

AU - Schupp, J.

AU - Van Ert, M.

AU - Keim, P.

N1 - Funding Information: We would like to thank the Northern Arizona University Cowden Endowment for Microbiology for support of this research.

PY - 2008/6

Y1 - 2008/6

N2 - The allelic identities of Single Nucleotide Repeat (SNR) markers in Bacillus anthracis are typically ascertained by DNA sequencing through the direct repeat. Here we describe a reproducible method for genotyping closely related isolates by using four SNR loci in a multiplex-PCR capillary electrophoresis system amenable to high-throughput analysis.

AB - The allelic identities of Single Nucleotide Repeat (SNR) markers in Bacillus anthracis are typically ascertained by DNA sequencing through the direct repeat. Here we describe a reproducible method for genotyping closely related isolates by using four SNR loci in a multiplex-PCR capillary electrophoresis system amenable to high-throughput analysis.

KW - Bacillus anthracis

KW - Capillary electrophoresis

KW - MLVA

KW - SNR

UR - https://www.scopus.com/pages/publications/43049134770

UR - https://www.scopus.com/pages/publications/43049134770#tab=citedBy

U2 - 10.1016/j.mimet.2007.11.014

DO - 10.1016/j.mimet.2007.11.014

M3 - Article

C2 - 18237793

AN - SCOPUS:43049134770

SN - 0167-7012

VL - 73

SP - 269

EP - 272

JO - Journal of Microbiological Methods

JF - Journal of Microbiological Methods

IS - 3

ER -

A high resolution four-locus multiplex single nucleotide repeat (SNR) genotyping system in Bacillus anthracis

Abstract

Keywords

ASJC Scopus subject areas

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