A high resolution four-locus multiplex single nucleotide repeat (SNR) genotyping system in Bacillus anthracis

L. J. Kenefic; J. Beaudry; C. Trim; L. Huynh; S. Zanecki; M. Matthews; J. Schupp; M. Van Ert; P. Keim

doi:10.1016/j.mimet.2007.11.014

A high resolution four-locus multiplex single nucleotide repeat (SNR) genotyping system in Bacillus anthracis

L. J. Kenefic, J. Beaudry, C. Trim, L. Huynh, S. Zanecki, M. Matthews, J. Schupp, M. Van Ert, P. Keim

Biological Sciences

Research output: Contribution to journal › Article › peer-review

32 Scopus citations

Abstract

The allelic identities of Single Nucleotide Repeat (SNR) markers in Bacillus anthracis are typically ascertained by DNA sequencing through the direct repeat. Here we describe a reproducible method for genotyping closely related isolates by using four SNR loci in a multiplex-PCR capillary electrophoresis system amenable to high-throughput analysis.

Original language	English (US)
Pages (from-to)	269-272
Number of pages	4
Journal	Journal of Microbiological Methods
Volume	73
Issue number	3
DOIs	https://doi.org/10.1016/j.mimet.2007.11.014
State	Published - Jun 2008

Keywords

Bacillus anthracis
Capillary electrophoresis
MLVA
SNR

ASJC Scopus subject areas

Microbiology
Molecular Biology
Microbiology (medical)

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10.1016/j.mimet.2007.11.014

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title = "A high resolution four-locus multiplex single nucleotide repeat (SNR) genotyping system in Bacillus anthracis",

abstract = "The allelic identities of Single Nucleotide Repeat (SNR) markers in Bacillus anthracis are typically ascertained by DNA sequencing through the direct repeat. Here we describe a reproducible method for genotyping closely related isolates by using four SNR loci in a multiplex-PCR capillary electrophoresis system amenable to high-throughput analysis.",

keywords = "Bacillus anthracis, Capillary electrophoresis, MLVA, SNR",

author = "Kenefic, {L. J.} and J. Beaudry and C. Trim and L. Huynh and S. Zanecki and M. Matthews and J. Schupp and {Van Ert}, M. and P. Keim",

note = "Funding Information: We would like to thank the Northern Arizona University Cowden Endowment for Microbiology for support of this research.",

year = "2008",

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doi = "10.1016/j.mimet.2007.11.014",

language = "English (US)",

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T1 - A high resolution four-locus multiplex single nucleotide repeat (SNR) genotyping system in Bacillus anthracis

AU - Kenefic, L. J.

AU - Beaudry, J.

AU - Trim, C.

AU - Huynh, L.

AU - Zanecki, S.

AU - Matthews, M.

AU - Schupp, J.

AU - Van Ert, M.

AU - Keim, P.

N1 - Funding Information: We would like to thank the Northern Arizona University Cowden Endowment for Microbiology for support of this research.

PY - 2008/6

Y1 - 2008/6

N2 - The allelic identities of Single Nucleotide Repeat (SNR) markers in Bacillus anthracis are typically ascertained by DNA sequencing through the direct repeat. Here we describe a reproducible method for genotyping closely related isolates by using four SNR loci in a multiplex-PCR capillary electrophoresis system amenable to high-throughput analysis.

AB - The allelic identities of Single Nucleotide Repeat (SNR) markers in Bacillus anthracis are typically ascertained by DNA sequencing through the direct repeat. Here we describe a reproducible method for genotyping closely related isolates by using four SNR loci in a multiplex-PCR capillary electrophoresis system amenable to high-throughput analysis.

KW - Bacillus anthracis

KW - Capillary electrophoresis

KW - MLVA

KW - SNR

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JF - Journal of Microbiological Methods

IS - 3

ER -

A high resolution four-locus multiplex single nucleotide repeat (SNR) genotyping system in Bacillus anthracis

Abstract

Keywords

ASJC Scopus subject areas

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